Characterization of Mutations That Allow p -Aminobenzoyl-Glutamate Utilization by Escherichia coli
نویسندگان
چکیده
منابع مشابه
Purification and characterization of the folate catabolic enzyme p-aminobenzoyl-glutamate hydrolase from Escherichia coli.
The abg locus of the Escherichia coli chromosome includes three genes encoding proteins (AbgA, AbgB, and AbgT) that enable uptake and utilization of the folate breakdown product, p-aminobenzoyl-glutamate (PABA-GLU). We report on the purification and characterization of the p-aminobenzoyl-glutamate hydrolase (PGH) holoenzyme encoded by abgA and abgB. One-step purification was accomplished using ...
متن کاملMutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose.
Wild-type strains of Escherichia coli are normally unable to metabolize cellobiose. However, cellobiose-positive (Cel(+)) mutants arise upon prolonged incubation on media containing cellobiose as the sole carbon source. We show that the Cel(+) derivatives carry two classes of mutations that act concertedly to alter the regulation of the chb operon involved in the utilization of N,N'-diacetylchi...
متن کاملComparison of Substrate Specificity of Escherichia Coli p-Aminobenzoyl-Glutamate Hydrolase with Pseudomonas Carboxypeptidase G
Reduced folic acid derivatives support biosynthesis of DNA, RNA and amino acids in bacteria as well as in eukaryotes, including humans. While the genes and steps for bacterial folic acid synthesis are known, those associated with folic acid catabolism are not well understood. A folate catabolite found in both humans and bacteria is p-aminobenzoyl-glutamate (PABA-GLU). The enzyme p-aminobenzoyl-...
متن کاملEscherichia coli abg genes enable uptake and cleavage of the folate catabolite p-aminobenzoyl-glutamate.
Escherichia coli AbgT was first identified as a structural protein enabling the growth of p-aminobenzoate auxotrophs on exogenous p-aminobenzoyl-glutamate (M. J. Hussein, J. M. Green, and B. P. Nichols, J. Bacteriol. 180:6260-6268, 1998). The abg region includes abgA, abgB, abgT, and ogt; these genes may be regulated by AbgR, a divergently transcribed LysR-type protein. Wild-type cells transfor...
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The utilization by the rat of yeast nucleotides labeled distinctively in the purine, sugar, and phosphate has been studied by Roll et al. (1, 2). They found that the purines of the 2’ and 3’ isomers of the purine nucleotides were both used to the same extent, but incorporation of the intact nucleotides was not observed. The ability of certain microorganisms to utilize efficiently the purine moi...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1998
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.180.23.6260-6268.1998